Code of Federal Regulations Title 40. Protection of Environment § 40.136.3, Table IC Identification of test procedures

Current as of January 02, 2025 | Updated by Findlaw Staff

Table IC—List of Approved Test Procedures for Non-Pesticide Organic Compounds
Parameter ¹	Method	EPA ²⁷	Standard methods	ASTM	Other
1. Acenaphthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
2. Acenaphthylene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
3. Acrolein	GC	603
	GC/MS	624.1,⁴ 1624B
4. Acrylonitrile	GC	603
	GC/MS	624.1,⁴ 1624B		․	O-4127-96.¹³
5. Anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
6. Benzene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
7. Benzidine	Spectrophoto-				See footnote ³, p.1.
	metric
	GC/MS	625.1,⁵ 1625B	6410 B-2000
	HPLC	605
8. Benzo(a)anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
9. Benzo(a)pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
10. Benzo(b)fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
11. Benzo(g,h,i)perylene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
12. Benzo(k)fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
13. Benzyl chloride	GC		․		See footnote ³, p. 130.
	GC/MS		․		See footnote ⁶, p. S102.
14. Butyl benzyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
15. bis(2-Chloroethoxy) methane	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
16. bis(2-Chloroethyl) ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
17. bis(2-Ethylhexyl) phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
18. Bromodichloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
19. Bromoform	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
20. Bromomethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
21. 4-Bromophenyl phenyl ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
22. Carbon tetrachloride	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
23. 4-Chloro-3-methyl phenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
24. Chlorobenzene	GC	601, 602	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
25. Chloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96.¹³
26. 2-Chloroethylvinyl ether	GC	601
	GC/MS	624.1, 1624B
27. Chloroform	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
28. Chloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
29. 2-Chloronaphthalene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
30. 2-Chlorophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
31. 4-Chlorophenyl phenyl ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
32. Chrysene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
33. Dibenzo(a,h)anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
34. Dibromochloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
35. 1,2-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote,⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
36. 1,3-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote ⁹, p. 27; O-4127-96.¹³
37. 1,4-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote,⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
38. 3,3'-Dichlorobenzidine	GC/MS	625.1, 1625B	6410 B-2000
	HPLC	605
39. Dichlorodifluoromethane	GC	601
	GC/MS		6200 C-2011		O-4127-96 ¹³, O-4436-16.¹⁴
40. 1,1-Dichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
41. 1,2-Dichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
42. 1,1-Dichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
43. trans-1,2-Dichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
44. 2,4-Dichlorophenol	GC	604	6420 B-2000.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
45. 1,2-Dichloropropane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
46. cis-1,3-Dichloropropene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
47. trans-1,3-Dichloropropene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
48. Diethyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
49. 2,4-Dimethylphenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
50. Dimethyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
51. Di-n-butyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹, p. 27.
52. Di-n-octyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27.
53. 2, 4-Dinitrophenol	GC	604	6420 B-2000		See footnote ⁹, p. 27.
	GC/MS	625.1, 1625B	6410 B-2000
54. 2,4-Dinitrotoluene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
55. 2,6-Dinitrotoluene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
56. Epichlorohydrin	GC		․		See footnote ³, p. 130.
	GC/MS		․		See footnote ⁶, p. S102.
57. Ethylbenzene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96,¹³ O-4436-16.¹⁴
58. Fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
59. Fluorene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
60. 1,2,3,4,6,7,8-Heptachloro-dibenzofuran	GC/MS	1613B
61. 1,2,3,4,7,8,9-Heptachloro-dibenzofuran	GC/MS	1613B
62. 1,2,3,4,6,7,8-Heptachloro-dibenzo-p-dioxin	GC/MS	1613B
63. Hexachlorobenzene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
64. Hexachlorobutadiene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96.¹³
65. Hexachlorocyclopentadiene	GC	612
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96.¹³
66. 1,2,3,4,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
67. 1,2,3,6,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
68. 1,2,3,7,8,9-Hexachloro-dibenzofuran	GC/MS	1613B
69. 2,3,4,6,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
70. 1,2,3,4,7,8-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
71. 1,2,3,6,7,8-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
72. 1,2,3,7,8,9-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
73. Hexachloroethane	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27; O-4127-96.¹³
74. Indeno(1,2,3-c,d) pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
75. Isophorone	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
76. Methylene chloride	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
77. 2-Methyl-4,6-dinitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
78. Naphthalene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005
79. Nitrobenzene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC		․	D4657-92 (98)
80. 2-Nitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
81. 4-Nitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
82. N-Nitrosodimethylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
83. N-Nitrosodi-n-propylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
84. N-Nitrosodiphenylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
85. Octachlorodibenzofuran	GC/MS	1613B.¹⁰
86. Octachlorodibenzo-p-dioxin	GC/MS	1613B.¹⁰
87. 2,2'-oxybis(1-chloropropane) ¹² [also known as bis(2-Chloro-1-methylethyl) ether]	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
88. PCB-1016	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
89. PCB-1221	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
90. PCB-1232	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
91. PCB-1242	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
92. PCB-1248	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
93. PCB-1254	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
94. PCB-1260	GC	608.3		․	See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
95. 1,2,3,7,8-Pentachloro-dibenzofuran	GC/MS	1613B
96. 2,3,4,7,8-Pentachloro-dibenzofuran	GC/MS	1613B
97. 1,2,3,7,8-Pentachloro-dibenzo-p-dioxin	GC/MS	1613B
98. Pentachlorophenol	GC	604	6420 B-2000		See footnote ³, p. 140.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
99. Phenanthrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
100. Phenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
101. Pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
102. 2,3,7,8-Tetrachloro-dibenzofuran	GC/MS	1613B.¹⁰
103. 2,3,7,8-Tetrachloro-dibenzo-p-dioxin	GC/MS	613, 625.1,^5a 1613B
104. 1,1,2,2-Tetrachloroethane	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96.¹³
105. Tetrachloroethene	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
106. Toluene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
107. 1,2,4-Trichlorobenzene	GC	612		․	See footnote ³, p. 130.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
108. 1,1,1-Trichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
109. 1,1,2-Trichloroethane	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
110. Trichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
111. Trichlorofluoromethane	GC	601	6200 C-2011
	GC/MS	624.1	6200 B-2011		O-4127-96.¹³
112. 2,4,6-Trichlorophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
113. Vinyl chloride	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
114. Nonylphenol	GC/MS		․	D7065-17
115. Bisphenol A (BPA)	GC/MS		․	D7065-17
116. p-tert-Octylphenol (OP)	GC/MS		․	D7065-17
117. Nonylphenol Monoethoxylate (NP1EO)	GC/MS		․	D7065-17
118. Nonylphenol Diethoxylate (NP2EO)	GC/MS		․	D7065-17
119. Adsorbable Organic Halides (AOX)	Adsorption and Coulometric Titration	1650.¹¹
120. Chlorinated Phenolics	In Situ Acetylation and GC/MS	1653.¹¹
Table IC notes:

¹ All parameters are expressed in micrograms per liter (μg/L) except for Method 1613B, in which the parameters are expressed in picograms per liter (pg/L).

² The full text of Methods 601-613, 1613B, 1624B, and 1625B are provided at appendix A, Test Procedures for Analysis of Organic Pollutants. The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at appendix B of this part, Definition and Procedure for the Determination of the Method Detection Limit. These methods are available at: https://www.epa.gov/cwa-methods as individual PDF files.

³ Methods for Benzidine: Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA.

⁴ Method 624.1 may be used for quantitative determination of acrolein and acrylonitrile, provided that the laboratory has documentation to substantiate the ability to detect and quantify these analytes at levels necessary to comply with any associated regulations. In addition, the use of sample introduction techniques other than simple purge-and-trap may be required. QC acceptance criteria from Method 603 should be used when analyzing samples for acrolein and acrylonitrile in the absence of such criteria in Method 624.1.

⁵ Method 625.1 may be extended to include benzidine, hexachlorocyclopentadiene, N-nitrosodimethylamine, N-nitrosodi-n-propylamine, and N-nitrosodiphenylamine. However, when they are known to be present, Methods 605, 607, and 612, or Method 1625B, are preferred methods for these compounds.

^5a Method 625.1 screening only.

⁶ Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).

⁷ Each analyst must make an initial, one-time demonstration of their ability to generate acceptable precision and accuracy with Methods 601-603, 1624B, and 1625B in accordance with procedures in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis must spike and analyze 10% (5% for Methods 624.1 and 625.1 and 100% for methods 1624B and 1625B) of all samples to monitor and evaluate laboratory data quality in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the quality control (QC) acceptance criteria in the pertinent method, analytical results for that parameter in the unspiked sample are suspect. The results should be reported but cannot be used to demonstrate regulatory compliance. If the method does not contain QC acceptance criteria, control limits of ± three standard deviations around the mean of a minimum of five replicate measurements must be used. These quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.

⁸ Organochlorine Pesticides and PCBs in Wastewater Using EmporeTM Disk. Revised October 28, 1994. 3M Corporation.

⁹ Method O-3116-87 is in Open File Report 93-125, Methods of Analysis by U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. USGS.

¹⁰ Analysts may use Fluid Management Systems, Inc. Power-Prep system in place of manual cleanup provided the analyst meets the requirements of Method 1613B (as specified in Section 9 of the method) and permitting authorities. Method 1613, Revision B, Tetra- through Octa-Chlorinated Dioxins and Furans by Isotope Dilution HRGC/HRMS. Revision B, 1994. U.S. EPA. The full text of this method is provided in appendix A to this part and at https://www.epa.gov/cwa-methods/approved-cwa-test-methods-organic-compounds.

¹¹ Method 1650, Adsorbable Organic Halides by Adsorption and Coulometric Titration. Revision C, 1997 U.S. EPA. Method 1653, Chlorinated Phenolics in Wastewater by In Situ Acetylation and GCMS. Revision A, 1997 U.S. EPA. The full text for both of these methods is provided at appendix A in part 430 of this chapter, The Pulp, Paper, and Paperboard Point Source Category.

¹² The compound was formerly inaccurately labeled as 2,2'-oxybis(2-chloropropane) and bis(2-chloroisopropyl) ether. Some versions of Methods 611, and 1625 inaccurately list the analyte as “bis(2-chloroisopropyl)ether,” but use the correct CAS number of 108-60-1.

¹³ Method O-4127-96, U.S. Geological Survey Open-File Report 97-829, Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of 86 volatile organic compounds in water by gas chromatography/mass spectrometry, including detections less than reporting limits,1998, USGS.

¹⁴ Method O-4436-16 U.S. Geological Survey Techniques and Methods, book 5, chap. B12, Determination of heat purgeable and ambient purgeable volatile organic compounds in water by gas chromatography/mass spectrometry, 2016, USGS.

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