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Code of Federal Regulations Title 40. Protection of Environment § 40.136.3, (b) to end Identification of test procedures
Current as of January 02, 2025 | Updated by Findlaw Staff
(b) Certain material is incorporated by reference into this section with the approval of the Director of the Federal Register under 5 U.S.C. 552(a) and 1 CFR part 51. All approved material may be inspected at EPA's Water Docket, EPA West, 1301 Constitution Avenue NW, Room 3334, Washington, DC 20004, (Telephone: 202–566–2426). It is also available for inspection at National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202–741–6030, or go to: http://www.archives.gov/federal-register/cfr/ibr-locations.html.
(1) Environmental Monitoring and Support Laboratory, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm or from: National Technical Information Service, 5285 Port Royal Road, Springfield, Virginia 22161
(i) Microbiological Methods for Monitoring the Environment, Water, and Wastes. 1978. EPA/600/8–78/017, Pub. No. PB–290329/A.S.
(A) Part III Analytical Methodology, Section B Total Coliform Methods, page 108. Table IA, Note 3; Table IH, Note 3.
(B) Part III Analytical Methodology, Section B Total Coliform Methods, 2.6.2 Two–Step Enrichment Procedure, page 111. Table IA, Note 3; Table IH, Note 3.
(C) Part III Analytical Methodology, Section B Total Coliform Methods, 4 Most Probable Number (MPN) Method, page 114. Table IA, Note 3; Table IH, Note 3.
(D) Part III Analytical Methodology, Section C Fecal Coliform Methods, 2 Direct Membrane Filter (MF) Method, page 124. Table IA, Note 3; Table IH, Note 3.
(E) Part III, Analytical Methodology, Section C Fecal Coliform Methods, 5 Most Probable Number (MPN) Method, page 132. Table IA, Note 3; Table IH, Note 3.
(F) Part III Analytical Methodology, Section D Fecal Streptococci, 2 Membrane Filter (MF) Method, page 136. Table IA, Note 3; Table IH, Note 3.
(G) Part III Analytical Methodology, Section D Fecal Streptococci, 4 Most Probable Number Method, page 139. Table IA, Note 3; Table IH, Note 3.
(H) Part III Analytical Methodology, Section D Fecal Streptococci, 5 Pour Plate Method, page 143. Table IA, Note 3; Table IH, Note 3.
(ii) [Reserved]
(2) Environmental Monitoring and Support Laboratory, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm.
(i) Method 300.1 (including Errata Cover Sheet, April 27, 1999), Determination of Inorganic Ions in Drinking Water by Ion Chromatography, Revision 1.0, 1997. Table IB, Note 52.
(ii) Method 551, Determination of Chlorination Disinfection Byproducts and Chlorinated Solvents in Drinking Water by Liquid–Liquid Extraction and Gas Chromatography With Electron–Capture Detection. 1990. Table IF.
(3) National Exposure Risk Laboratory–Cincinnati, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available from http://water.epa.gov/scitech/methods/cwa/index.cfm or from the National Technical Information Service (NTIS), 5285 Port Royal Road, Springfield, VA 22161. Telephone: 800–553–6847.
(i) Methods for the Determination of Inorganic Substances in Environmental Samples. August 1993. EPA/600/R–93/100, Pub. No. PB 94120821. Table IB, Note 52.
(A) Method 180.1, Determination of Turbidity by Nephelometry. Revision 2.0. Table IB, Note 52.
(B) Method 300.0, Determination of Inorganic Anions by Ion Chromatography. Revision 2.1. Table IB, Note 52.
(C) Method 335.4, Determination of Total Cyanide by Semi–Automated Colorimetry. Revision 1.0. Table IB, Notes 52 and 57.
(D) Method 350.1, Determination of Ammonium Nitrogen by Semi–Automated Colorimetry. Revision 2.0. Table IB, Notes 30 and 52.
(E) Method 351.2, Determination of Total Kjeldahl Nitrogen by Semi–Automated Colorimetry. Revision 2.0. Table IB, Note 52.
(F) Method 353.2, Determination of Nitrate–Nitrite Automated Colorimetry. Revision 2.0. Table IB, Note 52.
(G) Method 365.1, Determination of Phosphorus by Automated Colorimetry. Revision 2.0. Table IB, Note 52.
(H) Method 375.2, Determination of Sulfate by Automated Colorimetry. Revision 2.0. Table IB, Note 52.
(I) Method 410.4, Determination of Chemical Oxygen Demand by Semi–Automated Colorimetry. Revision 2.0. Table IB, Note 52.
(ii) Methods for the Determination of Metals in Environmental Samples, Supplement I. May 1994. EPA/600/R–94/111, Pub. No. PB 95125472. Table IB, Note 52.
(A) Method 200.7, Determination of Metals and Trace Elements in Water and Wastes by Inductively Coupled Plasma–Atomic Emission Spectrometry. Revision 4.4. Table IB, Note 52.
(B) Method 200.8, Determination of Trace Elements in Water and Wastes by Inductively Coupled Plasma Mass Spectrometry. Revision 5.3. Table IB, Note 52.
(C) Method 200.9, Determination of Trace Elements by Stabilized Temperature Graphite Furnace Atomic Absorption Spectrometry. Revision 2.2. Table IB, Note 52.
(D) Method 218.6, Determination of Dissolved Hexavalent Chromium in Drinking Water, Groundwater, and Industrial Wastewater Effluents by Ion Chromatography. Revision 3.3. Table IB, Note 52.
(E) Method 245.1, Determination of Mercury in Water by Cold Vapor Atomic Absorption Spectrometry. Revision 3.0. Table IB, Note 52.
(4) National Exposure Risk Laboratory–Cincinnati, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm.
(i) EPA Method 200.5, Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma–Atomic Emission Spectrometry. Revision 4.2, October 2003. EPA/600/R–06/115. Table IB, Note 68.
(ii) EPA Method 525.2, Determination of Organic Compounds in Drinking Water by Liquid–Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry. Revision 2.0, 1995. Table ID, Note 10.
(5) Office of Research and Development, Cincinnati OH. U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm or from ORD Publications, CERI, U.S. Environmental Protection Agency, Cincinnati OH 45268.
(i) Methods for Benzidine, Chlorinated Organic Compounds, Pentachlorophenol, and Pesticides in Water and Wastewater. 1978. Table IC, Note 3; Table ID, Note 3.
(ii) Methods for Chemical Analysis of Water and Wastes. March 1979. EPA–600/4–79–020. Table IB, Note 1.
(iii) Methods for Chemical Analysis of Water and Wastes. Revised March 1983. EPA–600/4–79–020. Table IB, Note 1.
(A) Method 120.1, Conductance, Specific Conductance, μmhos at 25 °C. Revision 1982. Table IB, Note 1.
(B) Method 130.1, Hardness, Total (mg/L as CaCO3), Colorimetric, Automated EDTA. Issued 1971. Table IB, Note 1.
(C) Method 150.2, pH, Continuous Monitoring (Electrometric). December 1982. Table IB, Note 1.
(D) Method 160.4, Residue, Volatile, Gravimetric, Ignition at 550 °C. Issued 1971. Table IB, Note 1.
(E) Method 206.5, Arsenic, Sample Digestion Prior to Total Arsenic Analysis by Silver Diethyldithiocarbamate or Hydride Procedures. Issued 1978. Table IB, Note 1.
(F) Method 231.2, Gold, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(G) Method 245.2, Mercury, Automated Cold Vapor Technique. Issued 1974. Table IB, Note 1.
(H) Method 252.2, Osmium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(I) Method 253.2, Palladium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(J) Method 255.2, Platinum, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(K) Method 265.2, Rhodium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(L) Method 279.2, Thallium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(M) Method 283.2, Titanium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(N) Method 289.2, Zinc, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.
(O) Method 310.2, Alkalinity, Colorimetric, Automated, Methyl Orange. Revision 1974. Table IB, Note 1.
(P) Method 351.1, Nitrogen, Kjeldahl, Total, Colorimetric, Automated Phenate. Revision 1978. Table IB, Note 1.
(Q) Method 352.1, Nitrogen, Nitrate, Colorimetric, Brucine. Issued 1971. Table IB, Note 1.
(R) Method 365.3, Phosphorus, All Forms, Colorimetric, Ascorbic Acid, Two Reagent. Issued 1978. Table IB, Note 1.
(S) Method 365.4, Phosphorus, Total, Colorimetric, Automated, Block Digestor AA II. Issued 1974. Table IB, Note 1.
(T) Method 410.3, Chemical Oxygen Demand, Titrimetric, High Level for Saline Waters. Revision 1978. Table IB, Note 1.
(U) Method 420.1, Phenolics, Total Recoverable, Spectrophotometric, Manual 4–AAP With Distillation. Revision 1978. Table IB, Note 1.
(iv) Prescribed Procedures for Measurement of Radioactivity in Drinking Water. 1980. EPA–600/4–80–032. Table IE.
(A) Method 900.0, Gross Alpha and Gross Beta Radioactivity. Table IE.
(B) Method 903.0, Alpha–Emitting iRadio Isotopes. Table IE.
(C) Method 903.1, Radium–226, Radon Emanation Technique. Table IE.
(D) Appendix B, Error and Statistical Calculations. Table IE.
(6) Office of Science and Technology, U.S. Environmental Protection Agency, Washington DC (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm.
(i) Method 1625C, Semivolatile Organic Compounds by Isotope Dilution GCMS. 1989. Table IF.
(ii) [Reserved]
(7) Office of Water, U.S. Environmental Protection Agency, Washington DC (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm or from National Technical Information Service, 5285 Port Royal Road, Springfield, Virginia 22161.
(i) Method 1631, Mercury in Water by Oxidation, Purge and Trap, and Cold Vapor Atomic Fluorescence Spectrometry. Revision E, August 2002. EPA–821–R–02–019, Pub. No. PB2002–108220. Table IB, Note 43.
(ii) Kelada–01, Kelada Automated Test Methods for Total Cyanide, Acid Dissociable Cyanide, and Thiocyanate. Revision 1.2, August 2001. EPA 821–B–01–009, Pub. No. PB 2001–108275. Table IB, Note 55.
(iii) In the compendium Analytical Methods for the Determination of Pollutants in Pharmaceutical Manufacturing Industry Wastewaters. July 1998. EPA 821–B–98–016, Pub. No. PB95201679. Table IF, Note 1.
(A) EPA Method 1666, Volatile Organic Compounds Specific to the Pharmaceutical Industry by Isotope Dilution GC/MS. Table IF, Note 1.
(B) EPA Method 1667, Formaldehyde, Isobutyraldehyde, and Furfural by Derivatization Followed by High Performance Liquid Chromatography. Table IF.
(C) Method 1671, Volatile Organic Compounds Specific to the Pharmaceutical Manufacturing Industry by GC/FID. Table IF.
(iv) Methods For The Determination of Nonconventional Pesticides In Municipal and Industrial Wastewater, Volume I. Revision I, August 1993. EPA 821–R–93–010A, Pub. No. PB 94121654. Tables ID, IG.
(A) Method 608.1, Organochlorine Pesticides. Table ID, Note 10; Table IG, Note 3.
(B) Method 608.2, Certain Organochlorine Pesticides. Table ID, Note 10; Table IG, Note 3.
(C) Method 614, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.
(D) Method 614.1, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.
(E) Method 615, Chlorinated Herbicides. Table ID, Note 10; Table IG, Note 3.
(F) Method 617, Organohalide Pesticides and PCBs. Table ID, Note 10; Table IG, Note 3.
(G) Method 619, Triazine Pesticides. Table ID, Note 10; Table IG, Note 3.
(H) Method 622, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.
(I) Method 622.1, Thiophosphate Pesticides. Table ID, Note 10; Table IG, Note 3.
(J) Method 627, Dinitroaniline Pesticides. Table ID, Note 10; Table IG, Notes 1 and 3.
(K) Method 629, Cyanazine. Table IG, Note 3.
(L) Method 630, Dithiocarbamate Pesticides. Table IG, Note 3.
(M) Method 630.1, Dithiocarbamate Pesticides. Table IG, Note 3.
(N) Method 631, Benomyl and Carbendazim. Table IG, Note 3.
(O) Method 632, Carbamate and Urea Pesticides. Table ID, Note 10; Table IG, Note 3.
(P) Method 632.1, Carbamate and Amide Pesticides. Table IG, Note 3.
(Q) Method 633, Organonitrogen Pesticides. Table IG, Note 3.
(R) Method 633.1, Neutral Nitrogen–Containing Pesticides. Table IG, Note 3.
(S) Method 637, MBTS and TCMTB. Table IG, Note 3.
(T) Method 644, Picloram. Table IG, Note 3.
(U) Method 645, Certain Amine Pesticides and Lethane. Table IG, Note 3.
(V) Method 1656, Organohalide Pesticides. Table ID, Note 10; Table IG, Notes 1 and 3.
(W) Method 1657, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.
(X) Method 1658, Phenoxy–Acid Herbicides. Table IG, Note 3.
(Y) Method 1659, Dazomet. Table IG, Note 3.
(Z) Method 1660, Pyrethrins and Pyrethroids. Table IG, Note 3.
(AA) Method 1661, Bromoxynil. Table IG, Note 3.
(BB) Ind–01. Methods EV–024 and EV–025, Analytical Procedures for Determining Total Tin and Triorganotin in Wastewater. Table IG, Note 3.
(v) Methods For The Determination of Nonconventional Pesticides In Municipal and Industrial Wastewater, Volume II. August 1993. EPA 821–R–93–010B, Pub. No. PB 94166311. Table IG.
(A) Method 200.9, Determination of Trace Elements by Stabilized Temperature Graphite Furnace Atomic Absorption Spectrometry. Table IG, Note 3.
(B) Method 505, Analysis of Organohalide Pesticides and Commercial Polychlorinated Biphenyl (PCB) Products in Water by Microextraction and Gas Chromatography. Table ID, Note 10; Table IG, Note 3.
(C) Method 507, The Determination of Nitrogen- and Phosphorus–Containing Pesticides in Water by Gas Chromatography with a Nitrogen–Phosphorus Detector. Table ID, Note 10; Table IG, Note 3.
(D) Method 508, Determination of Chlorinated Pesticides in Water by Gas Chromatography with an Electron Capture Detector. Table ID, Note 10; Table IG, Note 3.
(E) Method 515.1, Determination of Chlorinated Acids in Water by Gas Chromatography with an Electron Capture Detector. Table IG, Notes 2 and 3.
(F) Method 515.2, Determination of Chlorinated Acids in Water Using Liquid–Solid Extraction and Gas Chromatography with an Electron Capture Detector. Table IG, Notes 2 and 3.
(G) Method 525.1, Determination of Organic Compounds in Drinking Water by Liquids–Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry. Table ID, Note 10; Table IG, Note 3.
(H) Method 531.1, Measurement of N–Methylcarbamoyloximes and N–Methylcarbamates in Water by Direct Aqueous Injection HPLC with Post–Column Derivatization. Table ID, Note 10; Table IG, Note 3.
(I) Method 547, Determination of Glyphosate in Drinking Water by Direct–Aqueous–Injection HPLC, Post–Column Derivatization, and Fluorescence Detection. Table IG, Note 3.
(J) Method 548, Determination of Endothall in Drinking Water by Aqueous Derivatization, Liquid–Solid Extraction, and Gas Chromatography with Electron–Capture Detector. Table IG, Note 3.
(K) Method 548.1, Determination of Endothall in Drinking Water by Ion–Exchange Extraction, Acidic Methanol Methylation and Gas Chromatography/Mass Spectrometry. Table IG, Note 3.
(L) Method 553, Determination of Benzidines and Nitrogen–Containing Pesticides in Water by Liquid–Liquid Extraction or Liquid–Solid Extraction and Reverse Phase High Performance Liquid Chromatography/Particle Beam/Mass Spectrometry Table ID, Note 10; Table IG, Note 3.
(M) Method 555, Determination of Chlorinated Acids in Water by High Performance Liquid Chromatography With a Photodiode Array Ultraviolet Detector. Table IG, Note 3.
(vi) In the compendium Methods for the Determination of Organic Compounds in Drinking Water. Revised July 1991, December 1998. EPA–600/4–88–039, Pub. No. PB92–207703. Table IF.
(A) EPA Method 502.2, Volatile Organic Compounds in Water by Purge and Trap Capillary Column Gas Chromatography with Photoionization and Electrolytic Conductivity Detectors in Series. Table IF.
(B) [Reserved]
(vii) In the compendium Methods for the Determination of Organic Compounds in Drinking Water–Supplement II. August 1992. EPA–600/R–92–129, Pub. No. PB92–207703. Table IF.
(A) EPA Method 524.2, Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry. Table IF.
(B) [Reserved]
(viii) Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, Fifth Edition. October 2002. EPA 821–R–02–012, Pub. No. PB2002–108488. Table IA, Note 26.
(ix) Short–Term Methods for Measuring the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition. October 2002. EPA 821–R–02–013, Pub. No. PB2002–108489. Table IA, Note 27.
(x) Short–Term Methods for Measuring the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, Third Edition. October 2002. EPA 821–R–02–014, Pub. No. PB2002–108490. Table IA, Note 28.
(8) Office of Water, U.S. Environmental Protection Agency, Washington, DC (U.S. EPA). Available at https://www.epa.gov/cwa-methods.
(i) Method 245.7, Mercury in Water by Cold Vapor Atomic Fluorescence Spectrometry. Revision 2.0, February 2005. EPA–821–R–05–001. Table IB, Note 17.
(ii) Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane–Thermotolerant Escherichia coli Agar (mTEC). March 2010. EPA–621–R–10–002. Table IH, Note 19.
(iii) Method 1106.1: Enterococci in Water by Membrane Filtration Using membrane–Enterococcus–Esculin Iron Agar (mE–EIA). December 2009. EPA–621–R–09–015. Table IH, Note 23.
(iv) Method 1600: Enterococci in Water by Membrane Filtration Using membrane–Enterococcus Indoxyl-β–D–Glucoside Agar (mEI).September 2014. EPA–821–R–14–011. Table IA, Note 25; Table IH, Note 24.
(v) Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane–Thermotolerant Escherichia coli Agar (Modified mTEC).September 2014. EPA–821–R–14–010. Table IA, Note 22; Table IH, Note 20.
(vi) Method 1604: Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium). September 2002. EPA–821–R–02–024. Table IH, Note 21.
(vii) Method 1622: Cryptosporidium in Water by Filtration/IMS/FA. December 2005. EPA–821–R–05–001. Table IH, Note 25.
(viii) Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. December 2005. EPA–821–R–05–002. Table IH, Note 26.
(ix) Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA 816–R–12–001. January 2012. U.S. EPA, Table IH, Note 25.
(x) Method 1627, Kinetic Test Method for the Prediction of Mine Drainage Quality. December 2011. EPA–821–R–09–002. Table IB, Note 69.
(xi) Method 1664, n–Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n–Hexane Extractable Material (SGT–HEM; Nonpolar Material) by Extraction and Gravimetry. Revision A, February 1999. EPA–821–R–98–002. Table IB, Notes 38 and 42.
(xii) Method 1664, n–Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n–Hexane Extractable Material (SGT–HEM; Nonpolar Material) by Extraction and Gravimetry, Revision B, February 2010. EPA–821–R–10–001. Table IB, Notes 38 and 42.
(xiii) Method 1669, Sampling Ambient Water for Trace Metals at EPA Water Quality Criteria Levels. July 1996. Table IB, Note 43.
(xiv) Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple–Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium. September 2014. EPA–821–R–14–009.Table IA, Note 15.
(xv) Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple–Tube Fermentation using A–1 Medium. July 2006. EPA 821–R–06–013. Table IA, Note 20.
(xvi) Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport–Vassiliadis (MSRV) Medium. September 2014. EPA 821–R–14–012. Table IA, Note 23.
(9) American National Standards Institute, 1430 Broadway, New York NY 10018.
(i) ANSI. American National Standard on Photographic Processing Effluents. April 2, 1975. Table IB, Note 9.
(ii) [Reserved]
(10) American Public Health Association, 1015 15th Street NW., Washington, DC 20005. Standard Methods Online is available through the Standard Methods Web site (http://www.standardmethods.org).
(i) Standard Methods for the Examination of Water and Wastewater. 14th Edition, 1975. Table IB, Notes 17 and 27.
(ii) Standard Methods for the Examination of Water and Wastewater. 15th Edition, 1980, Table IB, Note 30; Table ID.
(iii) Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. Table IC, Note 6; Table ID, Note 6.
(iv) Standard Methods for the Examination of Water and Wastewater. 18th Edition, 1992. Tables IA, IB, IC, ID, IE, and IH.
(v) Standard Methods for the Examination of Water and Wastewater. 19th Edition, 1995. Tables IA, IB, IC, ID, IE, and IH.
(vi) Standard Methods for the Examination of Water and Wastewater. 20th Edition, 1998. Tables IA, IB, IC, ID, IE, and IH.
(vii) Standard Methods for the Examination of Water and Wastewater. 21st Edition, 2005. Table IB, Notes 17 and 27.
(viii) 2120, Color.2011. Table IB.
(ix) 2130, Turbidity.2011. Table IB.
(x) 2310, Acidity.2011. Table IB.
(xi) 2320, Alkalinity.2011. Table IB.
(xii) 2340, Hardness.2011. Table IB.
(xiii) 2510, Conductivity.2011. Table IB.
(xiv) 2540, solids. 2015. Table IB.
(xv) 2550, Temperature.2010. Table IB.
(xvi) 3111, Metals by Flame Atomic Absorption Spectrometry.2011. Table IB.
(xvii) 3112, Metals by Cold–Vapor Atomic Absorption Spectrometry.2011. Table IB.
(xviii) 3113, Metals by Electrothermal Atomic Absorption Spectrometry.2010. Table IB.
(xix) 3114, Arsenic and Selenium by Hydride Generation/Atomic Absorption Spectrometry.2011. Table IB.
(xx) 3120, Metals by Plasma Emission Spectroscopy. 2011. Table IB.
(xxi) 3125, Metals by Inductively Coupled Plasma–Mass Spectrometry.2011. Table IB.
(xxii) 3500–Al, Aluminum.2011. Table IB.
(xxiii) 3500–As, Arsenic.2011. Table IB.
(xxiv) 3500–Ca, Calcium.2011. Table IB.
(xxv) 3500–Cr, Chromium.2011. Table IB.
(xxvi) 3500–Cu, Copper.2011. Table IB.
(xxvii) 3500–Fe, Iron.2011. Table IB.
(xxviii) 3500–Pb, Lead.2011. Table IB.
(xxix) 3500–Mn, Manganese.2011. Table IB.
(xxx) 3500–K, Potassium.2011. Table IB.
(xxxi) 3500–Na, Sodium.2011. Table IB.
(xxxii) 3500–V, Vanadium.2011. Table IB.
(xxxiii) 3500–Zn, Zinc.2011. Table IB.
(xxxiv) 4110, Determination of Anions by Ion Chromatography.2011. Table IB.
(xxxv) 4140, Inorganic Anions by Capillary Ion Electrophoresis.2011. Table IB.
(xxxvi) 4500–B, Boron.2011. Table IB.
(xxxvii) 4500–Cl-, Chloride.2011. Table IB.
(xxxviii) 4500–Cl, Chlorine (Residual).2011. Table IB.
(xxxix) 4500–CN–, Cyanide. 2016. Table IB.
(xl) 4500–F-, Fluoride.2011. Table IB.
(xli) 4500–H+, pH Value.2011. Table IB.
(xlii) 4500–NH3, Nitrogen (Ammonia).2011. Table IB.
(xliii) 4500–NO2-, Nitrogen (Nitrite).2011. Table IB.
(xliv) 4500–NO3 -, Nitrogen (Nitrate). 2016. Table IB.
(xlv) 4500–Norg, Nitrogen (Organic).2011. Table IB.
(xlvi) 4500–O, Oxygen (Dissolved). 2016. Table IB.
(xlvii) 4500–P, Phosphorus.2011. Table IB.
(xlviii) 4500–SiO2, Silica.2011. Table IB.
(xlix) 4500–S2–, Sulfide.2011. Table IB.
(l) 4500–SO32–, Sulfite.2011. Table IB.
(li) 4500–SO42–, Sulfate.2011. Table IB.
(lii) 5210, Biochemical Oxygen Demand (BOD). 2016. Table IB.
(liii) 5220, Chemical Oxygen Demand (COD).2011. Table IB.
(liv) 5310, Total Organic Carbon (TOC). 2014. Table IB.
(lv) 5520, Oil and Grease. 2011. Table IB.
(lvi) 5530, Phenols.2010. Table IB.
(lvii) 5540, Surfactants.2011. Table IB.
(lviii) 6200, Volatile Organic Compounds.2011. Table IC.
(lix) 6410, Extractable Base/Neutrals and Acids. 2000. Tables IC, ID.
(lx) 6420, Phenols. 2000. Table IC.
(lxi) 6440, Polynuclear Aromatic Hydrocarbons.2005. Table IC.
(lxii) 6630, Organochlorine Pesticides.2007. Table ID.
(lxiii) 6640, Acidic Herbicide Compounds.2006. Table ID.
(lxiv) 7110, Gross Alpha and Gross Beta Radioactivity (Total, Suspended, and Dissolved). 2000. Table IE.
(lxv) 7500, Radium. 2001. Table IE.
(lxvi) 9213, Recreational Waters. 2007. Table IH.
(lxvii) 9221 Multiple–Tube Fermentation Technique for Members of the Coliform Group. 2014. Table IA, Notes 12 and 14; Table IH, Notes 10 and 12.
(lxviii) 9222, Membrane Filter Technique for Members of the Coliform Group. 2015. Table IA; Table IH, Note 17.
(lxix) 9223 Enzyme Substrate Coliform Test. 2016. Table IA; Table IH.
(lxx) 9230 Fecal Enterococcus/Streptococcus Groups. 2013. Table IA; Table IH.
(11) The Analyst, The Royal Society of Chemistry, RSC Publishing, Royal Society of Chemistry, Thomas Graham House, Science Park, Milton Road, Cambridge CB4 0WF, United Kingdom. (Also available from most public libraries.)
(i) Spectrophotometric Determination of Ammonia: A Study of a Modified Berthelot Reaction Using Salicylate and Dichloroisocyanurate. Krom, M.D. 105:305–316, April 1980. Table IB, Note 60.
(ii) [Reserved]
(12) Analytical Chemistry, ACS Publications, 1155 Sixteenth St. NW., Washington DC 20036. (Also available from most public libraries.)
(i) Spectrophotometric and Kinetics Investigation of the Berthelot Reaction for the Determination of Ammonia. Patton, C.J. and S.R. Crouch. 49(3):464–469, March 1977. Table IB, Note 60.
(ii) [Reserved]
(13) AOAC International, 481 North Frederick Avenue, Suite 500, Gaithersburg, MD 20877–2417.
(i) Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, 1998.
(A) 920.203, Manganese in Water, Persulfate Method. Table IB, Note 3.
(B) 925.54, Sulfate in Water, Gravimetric Method. Table IB, Note 3.
(C) 973.40, Specific Conductance of Water. Table IB, Note 3.
(D) 973.41, pH of Water. Table IB, Note 3.
(E) 973.43, Alkalinity of Water, Titrimetric Method. Table IB, Note 3.
(F) 973.44, Biochemical Oxygen Demand (BOD) of Water, Incubation Method. Table IB, Note 3.
(G) 973.45, Oxygen (Dissolved) in Water, Titrimetric Methods. Table IB, Note 3.
(H) 973.46, Chemical Oxygen Demand (COD) of Water, Titrimetric Methods. Table IB, Note 3.
(I) 973.47, Organic Carbon in Water, Infrared Analyzer Method. Table IB, Note 3.
(J) 973.48, Nitrogen (Total) in Water, Kjeldahl Method. Table IB, Note 3.
(K) 973.49, Nitrogen (Ammonia) in Water, Colorimetric Method. Table IB, Note 3.
(L) 973.50, Nitrogen (Nitrate) in Water, Brucine Colorimetric Method. Table IB, Note 3.
(M) 973.51, Chloride in Water, Mercuric Nitrate Method. Table IB, Note 3.
(N) 973.52, Hardness of Water. Table IB, Note 3.
(O) 973.53, Potassium in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.
(P) 973.54, Sodium in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.
(Q) 973.55, Phosphorus in Water, Photometric Method. Table IB, Note 3.
(R) 973.56, Phosphorus in Water, Automated Method. Table IB, Note 3.
(S) 974.27, Cadmium, Chromium, Copper, Iron, Lead, Magnesium, Manganese, Silver, Zinc in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.
(T) 977.22, Mercury in Water, Flameless Atomic Absorption Spectrophotometric Method. Table IB, Note 3.
(U) 991.15. Total Coliforms and Escherichia coli in Water Defined Substrate Technology (Colilert) Method. Table IA, Note 10; Table IH, Note 10.
(V) 993.14, Trace Elements in Waters and Wastewaters, Inductively Coupled Plasma–Mass Spectrometric Method. Table IB, Note 3.
(W) 993.23, Dissolved Hexavalent Chromium in Drinking Water, Ground Water, and Industrial Wastewater Effluents, Ion Chromatographic Method. Table IB, Note 3.
(X) 993.30, Inorganic Anions in Water, Ion Chromatographic Method. Table IB, Note 3.
(ii) [Reserved]
(14) Applied and Environmental Microbiology, American Society for Microbiology, 1752 N Street NW., Washington DC 20036. (Also available from most public libraries.)
(i) New Medium for the Simultaneous Detection of Total Coliforms and Escherichia coli in Water. Brenner, K.P., C.C. Rankin, Y.R. Roybal, G.N. Stelma, Jr., P.V. Scarpino, and A.P. Dufour. 59:3534–3544, November 1993. Table IH, Note 21.
(ii) [Reserved]
(15) ASTM International, 100 Barr Harbor Drive, P.O. Box C700, West Conshohocken, PA 19428–2959, or online at http://www.astm.org.
(i) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1994. Tables IA, IB, IC, ID, IE, and IH.
(ii) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1996. Tables IA, IB, IC, ID, IE, and IH.
(iii) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1999. Tables IA, IB, IC, ID, IE, and IH.
(iv) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 2000. Tables IA, IB, IC, ID, IE, and IH.
(v) ASTM D511–14, Standard Test Methods for Calcium and Magnesium in Water. November 2014. Table IB.
(vi) ASTM D512–12, Standard Test Methods for Chloride Ion in Water. July 2012. Table IB.
(vii) ASTM D515–88, Test Methods for Phosphorus in Water, March 1989. Table IB.
(viii) ASTM D516–16, Standard Test Method for Sulfate Ion in Water, June 2016. Table IB.
(ix) ASTM D858–17, Standard Test Methods for Manganese in Water. June 2017. Table IB.
(x) ASTM D859–16, Standard Test Method for Silica in Water. June 2016. Table IB.
(xi) ASTM D888–12, Standard Test Methods for Dissolved Oxygen in Water. March 2012. Table IB.
(xii) ASTM D1067–16, Standard Test Methods for Acidity or Alkalinity of Water. June 2016. Table IB.
(xiii) ASTM D1068–15, Standard Test Methods for Iron in Water. October 2015. Table IB.
(xiv) ASTM D1125–95 (Reapproved 1999), Standard Test Methods for Electrical Conductivity and Resistivity of Water. December 1995. Table IB.
(xv) ASTM D1126–17, Standard Test Method for Hardness in Water. December 2017. Table IB.
(xvi) ASTM D1179–16, Standard Test Methods for Fluoride Ion in Water. June 2016. Table IB.
(xvii) ASTM D1246–16, Standard Test Method for Bromide Ion in Water. June 2016. Table IB.
(xviii) ASTM D1252–06 (Reapproved 2012), Standard Test Methods for Chemical Oxygen Demand (Dichromate Oxygen Demand) of Water. June 2012. Table IB.
(xix) ASTM D1253–14, Standard Test Method for Residual Chlorine in Water. February 2014. Table IB.
(xx) ASTM D1293–99, Standard Test Methods for pH of Water. March 2000. Table IB.
(xxi) ASTM D1426–15, Standard Test Methods for Ammonia Nitrogen in Water. April 2015. Table IB.
(xxii) ASTM D1687–17, Standard Test Methods for Chromium in Water. July 2017. Table IB.
(xxiii) ASTM D1688–17, Standard Test Methods for Copper in Water. July 2017. Table IB.
(xxiv) ASTM D1691–17, Standard Test Methods for Zinc in Water. June 2017. Table IB.
(xxv) ASTM D1783–01 (Reapproved 2012), Standard Test Methods for Phenolic Compounds in Water. August 2012. Table IB.
(xxvi) ASTM D1886–14, Standard Test Methods for Nickel in Water. November 2014. Table IB.
(xxvii) ASTM D1889–00, Standard Test Method for Turbidity of Water. October 2000. Table IB.
(xxviii) ASTM D1890–96, Standard Test Method for Beta Particle Radioactivity of Water. April 1996. Table IE.
(xxix) ASTM D1943–96, Standard Test Method for Alpha Particle Radioactivity of Water. April 1996. Table IE.
(xxx) ASTM D1976–12, Standard Test Method for Elements in Water by Inductively–Coupled Argon Plasma Atomic Emission Spectroscopy.March 2012. Table IB.
(xxxi) ASTM D2036–09 (Reapproved 2015), Standard Test Methods for Cyanides in Water. July 2015. Table IB.
(xxxii) ASTM D2330–02, Standard Test Method for Methylene Blue Active Substances. August 2002. Table IB.
(xxxiii) ASTM D2460–97, Standard Test Method for Alpha–Particle–Emitting Isotopes of Radium in Water. October 1997. Table IE.
(xxxiv) ASTM D2972–15, Standard Tests Method for Arsenic in Water. March 2015. Table IB.
(xxxv) ASTM D3223–17, Standard Test Method for Total Mercury in Water. June 2017. Table IB.
(xxxvi) ASTM D3371–95, Standard Test Method for Nitriles in Aqueous Solution by Gas–Liquid Chromatography, February 1996. Table IF.
(xxxvii) ASTM D3373–17, Standard Test Method for Vanadium in Water. June 2017. Table IB.
(xxxviii) ASTM D3454–97, Standard Test Method for Radium–226 in Water. February 1998. Table IE.
(xxxix) ASTM D3557–17, Standard Test Method for Cadmium in Water. June 2017. Table IB.
(xl) ASTM D3558–15, Standard Test Method for Cobalt in Water. March 2015. Table IB.
(xli) ASTM D3559–15, Standard Test Methods for Lead in Water. October 2015. Table IB.
(xlii) ASTM D3590–17, Standard Test Methods for Total Kjeldahl Nitrogen in Water. June 2017. Table IB.
(xliii) ASTM D3645–15, Standard Test Methods for Beryllium in Water. March 2015. Table IB.
(xliv) ASTM D3695–95, Standard Test Method for Volatile Alcohols in Water by Direct Aqueous–Injection Gas Chromatography. April 1995. Table IF.
(xlv) ASTM D3859–15, Standard Test Methods for Selenium in Water. April 2015. Table IB.
(xlvi) ASTM D3867–16, Standard Test Method for Nitrite–Nitrate in Water. June 2016. Table IB.
(xlvii) ASTM D4190–15, Standard Test Method for Elements in Water by Direct- Current Plasma Atomic Emission Spectroscopy. March 2015. Table IB.
(xlviii) ASTM D4282–15, Standard Test Method for Determination of Free Cyanide in Water and Wastewater by Microdiffusion. July 2015. Table IB.
(xlix) ASTM D4327–17, Standard Test Method for Anions in Water by Suppressed Ion Chromatography. December 2017. Table IB.
(l) ASTM D4382–18, Standard Test Method for Barium in Water, Atomic Absorption Spectrophotometry, Graphite Furnace. May 2018. Table IB.
(li) ASTM D4657–92 (Reapproved 1998), Standard Test Method for Polynuclear Aromatic Hydrocarbons in Water. January 1993. Table IC.
(lii) ASTM D4658–15, Standard Test Method for Sulfide Ion in Water. April 2015. Table IB.
(liii) ASTM D4763–88 (Reapproved 2001), Standard Practice for Identification of Chemicals in Water by Fluorescence Spectroscopy. September 1988. Table IF.
(liv) ASTM D4839–03 (Reapproved 2017), Standard Test Method for Total Carbon and Organic Carbon in Water by Ultraviolet, or Persulfate Oxidation, or Both, and Infrared Detection. December 2017. Table IB.
(lv) ASTM D5257–17, Standard Test Method for Dissolved Hexavalent Chromium in Water by Ion Chromatography. December 2017. Table IB.
(lvi) ASTM D5259–92, Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure. October 1992. Table IH, Note 9.
(lvii) ASTM D5392–93, Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two–Step Membrane Filter Procedure. September 1993. Table IH, Note 9.
(lviii) ASTM D5673–16, Standard Test Method for Elements in Water by Inductively Coupled Plasma—Mass Spectrometry. February 2016. Table IB.
(lix) ASTM D5(19)907–13, Standard Test Method for Filterable Matter (Total Dissolved Solids) and Nonfilterable Matter (Total Suspended Solids) in Water. July 2013. Table IB.
(lx) ASTM D6503–99, Standard Test Method for Enterococci in Water Using Enterolert. April 2000. Table IA Note 9, Table IH, Note 9.
(lxi) ASTM. D6508–15, Standard Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. October 2015. Table IB, Note 54.
(lxii) ASTM. D6888–16, Standard Test Method for Available Cyanide with Ligand Displacement and Flow Injection Analysis (FIA) Utilizing Gas Diffusion Separation and Amperometric Detection. June 2016. Table IB, Note 59.
(lxiii) ASTM. D6919–17, Standard Test Method for Determination of Dissolved Alkali and Alkaline Earth Cations and Ammonium in Water and Wastewater by Ion Chromatography. June 2017. Table IB.
(lxiv) ASTM. D7065–17, Standard Test Method for Determination of Nonylphenol, Bisphenol A, p-tert–Octylphenol, Nonylphenol Monoethoxylate and Nonylphenol Diethoxylate in Environmental Waters by Gas Chromatography Mass Spectrometry. January 2018. Table IC.
(lxv) ASTM. D7237–15a, Standard Test Method for Free Cyanide with Flow Injection Analysis (FIA) Utilizing Gas Diffusion Separation and Amperometric Detection. June 2015. Table IB.
(lxvi) ASTM. D7284–13 (Reapproved 2017), Standard Test Method for Total Cyanide in Water by Micro Distillation followed by Flow Injection Analysis with Gas Diffusion Separation and Amperometric Detection. July 2017. Table IB.
(lxvii) ASTM. D7365–09a, Standard Practice for Sampling, Preservation, and Mitigating Interferences in Water Samples for Analysis of Cyanide. October 2009. Table II, Notes 5 and 6.
(lxviii) ASTM. D7511–12 (Reapproved 2017), Standard Test Method for Total Cyanide by Segmented Flow Injection Analysis, In–Line Ultraviolet Digestion and Amperometric Detection. July 2017. Table IB.
(lxix) ASTM. D7573–09 (Reapproved 2017), Standard Test Method for Total Carbon and Organic Carbon in Water by High Temperature Catalytic Combustion and Infrared Detection, February 2017. Table IB.
(lxx) ASTM D7781–14 Standard Test Method for Nitrate–Nitrite in Water by Nitrate Reductase, May 2014. Table IB.
(16) Bran & Luebbe Analyzing Technologies, Inc., Elmsford NY 10523.
(i) Industrial Method Number 378–75WA, Hydrogen Ion (pH) Automated Electrode Method, Bran & Luebbe (Technicon) Auto Analyzer II. October 1976. Table IB, Note 21.
(ii) [Reserved]
(17) CEM Corporation, P.O. Box 200, Matthews NC 28106–0200.
(i) Closed Vessel Microwave Digestion of Wastewater Samples for Determination of Metals. April 16, 1992. Table IB, Note 36.
(ii) [Reserved]
(18) Craig R. Chinchilla, 900 Jorie Blvd., Suite 35, Oak Brook IL 60523. Telephone: 630–645–0600.
(i) Nitrate by Discrete Analysis Easy (1–Reagent) Nitrate Method, (Colorimetric, Automated, 1 Reagent). Revision 1, November 12, 2011. Table IB, Note 62.
(ii) [Reserved]
(19) FIAlab Instruments, Inc., 2151 N. Northlake Way, Seattle, WA 98103. Telephone: 425–376–0450.
(i) Method 100, Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Fluorescence Detector Analysis, April 4, 2018. Table IB, Note 82.
(ii) [Reserved]
(20) Hach Company, P.O. Box 389, Loveland CO 80537.
(i) Method 8000, Chemical Oxygen Demand. Hach Handbook of Water Analysis. 1979. Table IB, Note 14.
(ii) Method 8008, 1,10–Phenanthroline Method using FerroVer Iron Reagent for Water. 1980. Table IB, Note 22.
(iii) Method 8009, Zincon Method for Zinc. Hach Handbook for Water Analysis. 1979. Table IB, Note 33.
(iv) Method 8034, Periodate Oxidation Method for Manganese. Hach Handbook for Water Analysis. 1979. Table IB, Note 23.
(v) Method 8506, Bicinchoninate Method for Copper. Hach Handbook of Water Analysis. 1979. Table IB, Note 19.
(vi) Method 8507, Nitrogen, Nitrite—Low Range, Diazotization Method for Water and Wastewater. 1979. Table IB, Note 25.
(vii) Method 10206, Hach Company TNTplus 835/836 Nitrate Method 10206, Spectrophotometric Measurement of Nitrate in Water and Wastewater. Revision 2.1, January 10, 2013. Table IB, Note 75.
(viii) Method 10242, Hach Company TNTplus 880 Total Kjeldahl Nitrogen Method 10242, Simplified Spectrophotometric Measurement of Total Kjeldahl Nitrogen in Water and Wastewater. Revision 1.1, January 10, 2013. Table IB, Note 76.
(ix) Hach Method 10360, Luminescence Measurement of Dissolved Oxygen in Water and Wastewater and for Use in the Determination of BOD5 and cBOD5. Revision 1.2, October 2011. Table IB, Note 63.
(x) m–ColiBlue24® Method, for total Coliforms and E. coli. Revision 2, 1999. Table IA, Note 18; Table IH, Note 17.
(21) IDEXX Laboratories Inc., One Idexx Drive, Westbrook ME 04092.
(i) Colilert. 2013. Table IA, Notes 17 and 18; Table IH, Notes 14, 15 and 16.
(ii) Colilert–18. 2013. Table IA, Notes 17 and 18; Table IH, Notes 14, 15 and 16.
(iii) Enterolert. 2013. Table IA, Note 24; Table IH, Note 12.
(iv) Quanti–Tray Insert and Most Probable Number (MPN) Table. 2013. Table IA, Note 18; Table IH, Notes 14 and 16.
(22) In–Situ Incorporated, 221 E. Lincoln Ave., Ft. Collins CO 80524. Telephone: 970–498–1500.
(i) In–Situ Inc. Method 1002–8–2009, Dissolved Oxygen Measurement by Optical Probe. 2009. Table IB, Note 64.
(ii) In–Situ Inc. Method 1003–8–2009, Biochemical Oxygen Demand (BOD) Measurement by Optical Probe. 2009. Table IB, Note 10.
(iii) In–Situ Inc. Method 1004–8–2009, Carbonaceous Biochemical Oxygen Demand (CBOD) Measurement by Optical Probe. 2009. Table IB, Note 35.
(23) Journal of Chromatography, Elsevier/North–Holland, Inc., Journal Information Centre, 52 Vanderbilt Avenue, New York NY 10164. (Also available from most public libraries.
(i) Direct Determination of Elemental Phosphorus by Gas–Liquid Chromatography. Addison, R.F. and R.G. Ackman. 47(3): 421–426, 1970. Table IB, Note 28.
(ii) [Reserved]
(24) Lachat Instruments, 6645 W. Mill Road, Milwaukee WI 53218, Telephone: 414–358–4200.
(i) QuikChem Method 10–204–00–1–X, Digestion and Distillation of Total Cyanide in Drinking and Wastewaters using MICRO DIST and Determination of Cyanide by Flow Injection Analysis. Revision 2.2, March 2005. Table IB, Note 56.
(ii) [Reserved]
(25) Leck Mitchell, Ph.D., P.E., 656 Independence Valley Dr., Grand Junction CO 81507. Telephone: 970–244–8661.
(i) Mitchell Method M5271, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Table IB, Note 66.
(ii) Mitchell Method M5331, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Table IB, Note 65.
(26) MACHEREY–NAGEL GmbH and Co., 2850 Emrick Blvd. Bethlehem, PA 18020. Telephone: 888–321–6224.
(i) Method 036/038 NANOCOLOR® COD LR/HR, Spectrophotometric Measurement of Chemical Oxygen Demand in Water and Wastewater, Revision 1.5, May 2018. Table IB, Note 83.
(ii) [Reserved]
(27) Micrology Laboratories, LLC, 1303 Eisenhower Drive, Goshen, IN 46526. Telephone: 574–533–3351.
(i) KwikCountTM EC Medium E. coli enzyme substrate test, Rapid Detection of E. coli in Beach Water By KwikCountTM EC Membrane Filtration. 2014. Table IH, Notes 28 and 29.
(ii) [Reserved]
(28) National Council of the Paper Industry for Air and Stream Improvements, Inc. (NCASI), 260 Madison Avenue, New York NY 10016.
(i) NCASI Method TNTP–W10900, Total Nitrogen and Total Phophorus in Pulp and Paper Biologically Treated Effluent by Alkaline Persulfate Digestion. June 2011. Table IB, Note 77.
(ii) NCASI Technical Bulletin No. 253, An Investigation of Improved Procedures for Measurement of Mill Effluent and Receiving Water Color. December 1971. Table IB, Note 18.
(iii) NCASI Technical Bulletin No. 803, An Update of Procedures for the Measurement of Color in Pulp Mill Wastewaters. May 2000. Table IB, Note 18.
(29) The Nitrate Elimination Co., Inc. (NECi), 334 Hecla St., Lake Linden NI 49945.
(i) NECi Method N07–0003, Method for Nitrate Reductase Nitrate–Nitrogen Analysis. Revision 9.0. March 2014. Table IB, Note 73.
(ii) [Reserved]
(30) Oceanography International Corporation, 512 West Loop, P.O. Box 2980, College Station TX 77840.
(i) OIC Chemical Oxygen Demand Method. 1978. Table IB, Note 13.
(ii) [Reserved]
(31) OI Analytical, Box 9010, College Station TX 77820–9010.
(i) Method OIA–1677–09, Available Cyanide by Ligand Exchange and Flow Injection Analysis (FIA). Copyright 2010. Table IB, Note 59.
(ii) Method PAI–DK01, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Titrimetric Detection. Revised December 22, 1994. Table IB, Note 39.
(iii) Method PAI–DK02, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Colorimetric Detection. Revised December 22, 1994. Table IB, Note 40.
(iv) Method PAI–DK03, Nitrogen, Total Kjeldahl, Block Digestion, Automated FIA Gas Diffusion. Revised December 22, 1994. Table IB, Note 41.
(32) ORION Research Corporation, 840 Memorial Drive, Cambridge, Massachusetts 02138.
(i) ORION Research Instruction Manual, Residual Chlorine Electrode Model 97–70. 1977. Table IB, Note 16.
(ii) [Reserved]
(33) Technicon Industrial Systems, Tarrytown NY 10591.
(i) Industrial Method Number 379–75WE Ammonia, Automated Electrode Method, Technicon Auto Analyzer II. February 19, 1976. Table IB, Note 7.
(ii) [Reserved]
(34) Thermo Jarrell Ash Corporation, 27 Forge Parkway, Franklin MA 02038.
(i) Method AES0029. Direct Current Plasma (DCP) Optical Emission Spectrometric Method for Trace Elemental Analysis of Water and Wastes. 1986, Revised 1991. Table IB, Note 34.
(ii) [Reserved]
(35) Thermo Scientific, 166 Cummings Center, Beverly MA 01915. Telephone: 1–800–225–1480. www.thermoscientific.com.
(i) Thermo Scientific Orion Method AQ4500, Determination of Turbidity by Nephelometry. Revision 5, March 12, 2009. Table IB, Note 67.
(ii) [Reserved]
(36) 3M Corporation, 3M Center Building 220–9E–10, St. Paul MN 55144–1000.
(i) Organochlorine Pesticides and PCBs in Wastewater Using EmporeTM Disk” Test Method 3M 0222. Revised October 28, 1994. Table IC, Note 8; Table ID, Note 8.
(ii) [Reserved]
(37) Timberline Instruments, LLC, 1880 South Flatiron Ct., Unit I, Boulder CO 80301.
(i) Timberline Amonia–001, Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Conductivity Cell Analysis. June 24, 2011. Table IB, Note 74.
(ii) [Reserved]
(38) U.S. Geological Survey (USGS), U.S. Department of the Interior, Reston, Virginia. Available from USGS Books and Open–File Reports (OFR) Section, Federal Center, Box 25425, Denver, CO 80225.
(i) Colorimetric determination of nitrate plus nitrite in water by enzymatic reduction, automated discrete analyzer methods. U.S. Geological Survey Techniques and Methods, Book 5—Laboratory Analysis, Section B—Methods of the National Water Quality Laboratory, Chapter 8. 2011. Table IB, Note 72.
(ii) Determination of Heat Purgeable and Ambient Purgeable Volatile Organic Compounds in Water by Gas Chromatography/Mass Spectrometry. Chapter 12 of Section B, Methods of the National Water Quality Laboratory, of Book 5, Laboratory Analysis. 2016.
(iii) Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, editors, Techniques of Water–Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1979. Table IB, Note 8.
(iv) Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, Techniques of Water–Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1989. Table IB, Notes 2 and 79.
(v) Methods for the Determination of Organic Substances in Water and Fluvial Sediments. Techniques of Water–Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A3. 1987. Table IB, Note 24; Table ID, Note 4.
(vi) OFR 76–177, Selected Methods of the U.S. Geological Survey of Analysis of Wastewaters. 1976. Table IE, Note 2.
(vii) OFR 91–519, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Organonitrogen Herbicides in Water by Solid–Phase Extraction and Capillary–Column Gas Chromatography/Mass Spectrometry With Selected–Ion Monitoring. 1992. Table ID, Note 14.
(viii) OFR 92–146, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Total Phosphorus by a Kjeldahl Digestion Method and an Automated Colorimetric Finish That Includes Dialysis. 1992. Table IB, Note 48.
(ix) OFR 93–125, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. Table IB, Note 51 and 80; Table IC, Note 9.
(x) OFR 93–449, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Chromium in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1993. Table IB, Note 46.
(xi) OFR 94–37, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Triazine and Other Nitrogen-containing Compounds by Gas Chromatography with Nitrogen Phosphorus Detectors. 1994. Table ID, Note 9.
(xii) OFR 95–181, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by C–18 Solid–Phase Extraction and Capillary–Column Gas Chromatography/Mass Spectrometry With Selected–Ion Monitoring. 1995. Table ID, Note 11.
(xiii) OFR 97–198, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Molybdenum in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1997. Table IB, Note 47.
(xiv) OFR 97–829, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory— Determination of 86 Volatile Organic Compounds in Water by Gas Chromatography/Mass Spectrometry, Including Detections Less Than Reporting Limits. 1999. Table IC, Note 13.
(xv) OFR 98–165, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Elements in Whole–Water Digests Using Inductively Coupled Plasma–Optical Emission Spectrometry and Inductively Coupled Plasma–Mass Spectrometry. 1998. Table IB, Notes 50 and 81.
(xvi) OFR 98–639, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Arsenic and Selenium in Water and Sediment by Graphite Furnace—Atomic Absorption Spectrometry. 1999. Table IB, Note 49.
(xvii) OFR 00–170, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Ammonium Plus Organic Nitrogen by a Kjeldahl Digestion Method and an Automated Photometric Finish that Includes Digest Cleanup by Gas Diffusion. 2000. Table IB, Note 45.
(xviii) Techniques and Methods Book 5–B1, Determination of Elements in Natural–Water, Biota, Sediment and Soil Samples Using Collision/Reaction Cell Inductively Coupled Plasma–Mass Spectrometry. Chapter 1, Section B, Methods of the National Water Quality Laboratory, Book 5, Laboratory Analysis. 2006. Table IB, Note 70.
(xix) U.S. Geological Survey Techniques of Water–Resources Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. Table IA, Note 4; Table IH, Note 4.
(xx) Water–Resources Investigation Report 01–4098, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Moderate–Use Pesticides and Selected Degradates in Water by C–18 Solid–Phase Extraction and Gas Chromatography/Mass Spectrometry. 2001. Table ID, Note 13.
(xxi) Water–Resources Investigations Report 01–4132, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Organic Plus Inorganic Mercury in Filtered and Unfiltered Natural Water With Cold Vapor–Atomic Fluorescence Spectrometry. 2001. Table IB, Note 71.
(xxii) Water–Resources Investigation Report 01–4134, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by Graphitized Carbon–Based Solid–Phase Extraction and High–Performance Liquid Chromatography/Mass Spectrometry. 2001. Table ID, Note 12.
(xxiii) Water Temperature—Influential Factors, Field Measurement and Data Presentation, Techniques of Water–Resources Investigations of the U.S. Geological Survey, Book 1, Chapter D1. 1975. Table IB, Note 32.
(39) Waters Corporation, 34 Maple Street, Milford MA 01757, Telephone: 508–482–2131, Fax: 508–482–3625.
(i) Method D6508, Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. Revision 2, December 2000. Table IB, Note 54.
(ii) [Reserved]
(c) Under certain circumstances, the Director may establish limitations on the discharge of a parameter for which there is no test procedure in this part or in 40 CFR parts 405 through 499. In these instances the test procedure shall be specified by the Director.
(d) Under certain circumstances, the Administrator may approve additional alternate test procedures for nationwide use, upon recommendation by the Alternate Test Procedure Program Coordinator, Washington, DC.
(e) Sample preservation procedures, container materials, and maximum allowable holding times for parameters are cited in Tables IA, IB, IC, ID, IE, IF, IG, and IH are prescribed in Table II. Information in the table takes precedence over information in specific methods or elsewhere. Any person may apply for a change from the prescribed preservation techniques, container materials, and maximum holding times applicable to samples taken from a specific discharge. Applications for such limited use changes may be made by letters to the Regional Alternative Test Procedure (ATP) Program Coordinator or the permitting authority in the Region in which the discharge will occur. Sufficient data should be provided to assure such changes in sample preservation, containers or holding times do not adversely affect the integrity of the sample. The Regional ATP Coordinator or permitting authority will review the application and then notify the applicant and the appropriate State agency of approval or rejection of the use of the alternate test procedure. A decision to approve or deny any request on deviations from the prescribed Table II requirements will be made within 90 days of receipt of the application by the Regional Administrator. An analyst may not modify any sample preservation and/or holding time requirements of an approved method unless the requirements of this section are met.
|
Table II—Required Containers, Preservation Techniques, and Holding Times |
|||
|---|---|---|---|
|
Parameter number/name |
Container 1 |
Maximum holding time 4 |
|
|
Table IA—Bacterial Tests |
|||
|
1-4. Coliform, total, fecal, and E. coli |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
|
|
5. Fecal streptococci |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
6. Enterococci |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
7. Salmonella |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
Table IA—Aquatic Toxicity Tests |
|||
|
8-11. Toxicity, acute and chronic |
P, FP, G |
Cool, ≤6 °C 16 |
36 hours. |
|
Table IB—Inorganic Tests |
|||
|
1. Acidity |
P, FP, G |
Cool, ≤6 °C 18 |
14 days. |
|
2. Alkalinity |
P, FP, G |
Cool, ≤6 °C 18 |
14 days. |
|
4. Ammonia |
P, FP, G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
9. Biochemical oxygen demand |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
10. Boron |
P, FP, or Quartz |
HNO3 to pH <2 |
6 months. |
|
11. Bromide |
P, FP, G |
None required |
28 days. |
|
14. Biochemical oxygen demand, carbonaceous |
P, FP G |
Cool, ≤6 °C 18 |
48 hours. |
|
15. Chemical oxygen demand |
P, FP, G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
16. Chloride |
P, FP, G |
None required |
28 days. |
|
17. Chlorine, total residual |
P, G |
None required |
Analyze within 15 minutes. |
|
21. Color |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
23-24. Cyanide, total or available (or CATC) and free |
P, FP, G |
Cool, ≤6 °C 18, NaOH to pH >10 56, reducing agent if oxidizer present |
14 days. |
|
25. Fluoride |
P |
None required |
28 days. |
|
27. Hardness |
P, FP, G |
HNO3 or H2SO4 to pH <2 |
6 months. |
|
28. Hydrogen ion (pH) |
P, FP, G |
None required |
Analyze within 15 minutes. |
|
31, 43. Kjeldahl and organic N |
P, FP, G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
Table IB—Metals 7 |
|||
|
18. Chromium VI |
P, FP, G |
28 days. |
|
|
35. Mercury (CVAA) |
P, FP, G |
HNO3 to pH <2 |
28 days. |
|
35. Mercury (CVAFS) |
FP, G; and FP-lined cap 17 |
5 mL/L 12N HCl or 5 mL/L BrCl 17 |
90 days.17 |
|
3, 5-8, 12, 13, 19, 20, 22, 26, 29, 30, 32-34, 36, 37, 45, 47, 51, 52, 58-60, 62, 63, 70-72, 74, 75. Metals, except boron, chromium VI, and mercury |
P, FP, G |
HNO3 to pH <2, or at least 24 hours prior to analysis 19 |
6 months. |
|
38. Nitrate |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
39. Nitrate-nitrite |
P, FP, G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
40. Nitrite |
P, FP, G |
Cool, ≤6 °C d18 |
48 hours. |
|
41. Oil and grease |
G |
Cool to ≤6 °C 18, HCl or H2SO4 to pH <2 |
28 days. |
|
42. Organic Carbon |
P, FP, G |
Cool to ≤6 °C 18, HCl, H2SO4, or H3PO4to pH <2 |
28 days. |
|
44. Orthophosphate |
P, FP, G |
Filter within 15 minutes; Analyze within 48 hours. |
|
|
46. Oxygen, Dissolved Probe |
G, Bottle and top |
None required |
Analyze within 15 minutes. |
|
47. Winkler |
G, Bottle and top |
Fix on site and store in dark |
8 hours. |
|
48. Phenols |
G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
49. Phosphorus (elemental) |
G |
Cool, ≤6 °C 18 |
48 hours. |
|
50. Phosphorus, total |
P, FP, G |
Cool, ≤6 °C 18, H2SO4 to pH <2 |
28 days. |
|
53. Residue, total |
P, FP, G |
Cool, ≤6 °C 18 |
7 days. |
|
54. Residue, Filterable (TDS) |
P, FP, G |
Cool, ≤6 °C 18 |
7 days. |
|
55. Residue, Nonfilterable (TSS) |
P, FP, G |
Cool, ≤6 °C 18 |
7 days. |
|
56. Residue, Settleable |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
57. Residue, Volatile |
P, FP, G |
Cool, ≤6 °C 18 |
7 days. |
|
61. Silica |
P or Quartz |
Cool, ≤6 °C 18 |
28 days. |
|
64. Specific conductance |
P, FP, G |
Cool, ≤6 °C 18 |
28 days. |
|
65. Sulfate |
P, FP, G |
Cool, ≤6 °C 18 |
28 days. |
|
66. Sulfide |
P, FP, G |
Cool, ≤6 °C 18, add zinc acetate plus sodium hydroxide to pH >9 |
7 days. |
|
67. Sulfite |
P, FP, G |
None required |
Analyze within 15 minutes. |
|
68. Surfactants |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
69. Temperature |
P, FP, G |
None required |
Analyze within 15 minutes. |
|
73. Turbidity |
P, FP, G |
Cool, ≤6 °C 18 |
48 hours. |
|
Table IC—Organic Tests 8 |
|||
|
13, 18-20, 22, 24, 25, 27, 28, 34-37, 39-43, 45-47, 56, 76, 104, 105, 108-111, 113. Purgeable Halocarbons |
G, FP-lined septum |
14 days.9 |
|
|
26. 2-Chloroethylvinyl ether |
G, FP-lined septum |
14 days. |
|
|
6, 57, 106. Purgeable aromatic hydrocarbons |
G, FP-lined septum |
14 days.9 |
|
|
3, 4. Acrolein and acrylonitrile |
G, FP-lined septum |
14 days.10 |
|
|
23, 30, 44, 49, 53, 77, 80, 81, 98, 100, 112. Phenols 11 |
G, FP-lined cap |
Cool, ≤6 °C 18, 0.008% Na2S2O3 |
7 days until extraction, 40 days after extraction. |
|
G, FP-lined cap |
7 days until extraction.13 |
||
|
14, 17, 48, 50-52. Phthalate esters 11 |
G, FP-lined cap |
Cool, ≤6 °C 18 |
7 days until extraction, 40 days after extraction. |
|
G, FP-lined cap |
7 days until extraction, 40 days after extraction. |
||
|
88-94. PCBs 11 |
G, FP-lined cap |
Cool, ≤6 °C 18 |
1 year until extraction, 1 year after extraction. |
|
54, 55, 75, 79. Nitroaromatics and isophorone 11 |
G, FP-lined cap |
7 days until extraction, 40 days after extraction. |
|
|
1, 2, 5, 8-12, 32, 33, 58, 59, 74, 78, 99, 101. Polynuclear aromatic hydrocarbons 11 |
G, FP-lined cap |
7 days until extraction, 40 days after extraction. |
|
|
15, 16, 21, 31, 87. Haloethers 11 |
G, FP-lined cap |
7 days until extraction, 40 days after extraction. |
|
|
29, 35-37, 63-65, 73, 107. Chlorinated hydrocarbons 11 |
G, FP-lined cap |
Cool, ≤6 °C 18 |
7 days until extraction, 40 days after extraction. |
|
60-62, 66-72, 85, 86, 95-97, 102, 103. CDDs/CDFs 11 |
G |
See footnote 11 |
See footnote 11. |
|
Aqueous Samples: Field and Lab Preservation |
G |
1 year. |
|
|
Solids and Mixed-Phase Samples: Field Preservation |
G |
Cool, ≤6 °C 18 |
7 days. |
|
Tissue Samples: Field Preservation |
G |
Cool, ≤6 °C 18 |
24 hours. |
|
Solids, Mixed-Phase, and Tissue Samples: Lab Preservation |
G |
Freeze, ≤-10 °C |
1 year. |
|
114-118. Alkylated phenols |
G |
Cool, <6 °C, H2SO4 to pH <2 |
28 days until extraction, 40 days after extraction. |
|
119. Adsorbable Organic Halides (AOX) |
G |
Cool, <6 °C, 0.008% Na2S2O3, HNO3 to pH <2 |
Hold at least 3 days, but not more than 6 months. |
|
120. Chlorinated Phenolics |
G, FP-lined cap |
Cool, <6 °C, 0.008% Na2S2O3, H2SO4 to pH <2 |
30 days until acetylation, 30 days after acetylation. |
|
Table ID—Pesticides Tests |
|||
|
1-70. Pesticides 11 |
G, FP-lined cap |
7 days until extraction, 40 days after extraction. |
|
|
Table IE—Radiological Tests |
|||
|
1-5. Alpha, beta, and radium |
P, FP, G |
HNO3 to pH <2 |
6 months. |
|
Table IH—Bacterial Tests |
|||
|
1, 2. Coliform, total, fecal |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
3.E. coli |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
4. Fecal streptococci |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
5. Enterococci |
PA, G |
Cool, <10 °C, 0.008% Na2S2O3 5 |
8 hours.22 |
|
Table IH—Protozoan Tests |
|||
|
6. Cryptosporidium |
LDPE; field filtration |
1-10 °C |
96 hours.21 |
|
7. Giardia |
LDPE; field filtration |
1-10 °C |
96 hours.21 |
1 ”P” is for polyethylene; “FP” is fluoropolymer (polytetrafluoroethylene [PTFE]; Teflon®), or other fluoropolymer, unless stated otherwise in this Table II; “G” is glass; “PA” is any plastic that is made of a sterilizable material (polypropylene or other autoclavable plastic); “LDPE” is low density polyethylene.
2 Except where noted in this Table II and the method for the parameter, preserve each grab sample within 15 minutes of collection. For a composite sample collected with an automated sample (e.g., using a 24-hour composite sample; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), refrigerate the sample at ≤6 °C during collection unless specified otherwise in this Table II or in the method(s). For a composite sample to be split into separate aliquots for preservation and/or analysis, maintain the sample at ≤6 °C, unless specified otherwise in this Table II or in the method(s), until collection, splitting, and preservation is completed. Add the preservative to the sample container prior to sample collection when the preservative will not compromise the integrity of a grab sample, a composite sample, or aliquot split from a composite sample within 15 minutes of collection. If a composite measurement is required but a composite sample would compromise sample integrity, individual grab samples must be collected at prescribed time intervals (e.g., 4 samples over the course of a day, at 6-hour intervals). Grab samples must be analyzed separately and the concentrations averaged. Alternatively, grab samples may be collected in the field and composited in the laboratory if the compositing procedure produces results equivalent to results produced by arithmetic averaging of results of analysis of individual grab samples. For examples of laboratory compositing procedures, see EPA Method 1664 Rev. A (oil and grease) and the procedures at 40 CFR 141.24(f)(14)(iv) and (v) (volatile organics).
3 When any sample is to be shipped by common carrier or sent via the U.S. Postal Service, it must comply with the Department of Transportation Hazardous Materials Regulations (49 CFR part 172). The person offering such material for transportation is responsible for ensuring such compliance. For the preservation requirement of Table II, the Office of Hazardous Materials, Materials Transportation Bureau, Department of Transportation has determined that the Hazardous Materials Regulations do not apply to the following materials: Hydrochloric acid (HCl) in water solutions at concentrations of 0.04% by weight or less (pH about 1.96 or greater; Nitric acid (HNO3) in water solutions at concentrations of 0.15% by weight or less (pH about 1.62 or greater); Sulfuric acid (H2SO4) in water solutions at concentrations of 0.35% by weight or less (pH about 1.15 or greater); and Sodium hydroxide (NaOH) in water solutions at concentrations of 0.080% by weight or less (pH about 12.30 or less).
4 Samples should be analyzed as soon as possible after collection. The times listed are the maximum times that samples may be held before the start of analysis and still be considered valid. Samples may be held for longer periods only if the permittee or monitoring laboratory have data on file to show that, for the specific types of samples under study, the analytes are stable for the longer time, and has received a variance from the Regional ATP Coordinator under § 136.3(e). For a grab sample, the holding time begins at the time of collection. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), the holding time begins at the time of the end of collection of the composite sample. For a set of grab samples composited in the field or laboratory, the holding time begins at the time of collection of the last grab sample in the set. Some samples may not be stable for the maximum time period given in the table. A permittee or monitoring laboratory is obligated to hold the sample for a shorter time if it knows that a shorter time is necessary to maintain sample stability. See 136.3(e) for details. The date and time of collection of an individual grab sample is the date and time at which the sample is collected. For a set of grab samples to be composited, and that are all collected on the same calendar date, the date of collection is the date on which the samples are collected. For a set of grab samples to be composited, and that are collected across two calendar dates, the date of collection is the dates of the two days; e.g., November 14-15. For a composite sample collected automatically on a given date, the date of collection is the date on which the sample is collected. For a composite sample collected automatically, and that is collected across two calendar dates, the date of collection is the dates of the two days; e.g., November 14-15. For static-renewal toxicity tests, each grab or composite sample may also be used to prepare test solutions for renewal at 24 h, 48 h, and/or 72 h after first use, if stored at 0-6 °C, with minimum head space.
5 ASTM D7365-09a specifies treatment options for samples containing oxidants (e.g., chlorine) for cyanide analyses. Also, Section 9060A of Standard Methods for the Examination of Water and Wastewater (23rd edition) addresses dechlorination procedures for microbiological analyses.
6 Sampling, preservation and mitigating interferences in water samples for analysis of cyanide are described in ASTM D7365-09a (15). There may be interferences that are not mitigated by the analytical test methods or D7365-09a (15). Any technique for removal or suppression of interference may be employed, provided the laboratory demonstrates that it more accurately measures cyanide through quality control measures described in the analytical test method. Any removal or suppression technique not described in D7365-09a (15) or the analytical test method must be documented along with supporting data.
7 For dissolved metals, filter grab samples within 15 minutes of collection and before adding preservatives. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), filter the sample within 15 minutes after completion of collection and before adding preservatives. If it is known or suspected that dissolved sample integrity will be compromised during collection of a composite sample collected automatically over time (e.g., by interchange of a metal between dissolved and suspended forms), collect and filter grab samples to be composited (footnote 2) in place of a composite sample collected automatically.
8 Guidance applies to samples to be analyzed by GC, LC, or GC/MS for specific compounds.
9 If the sample is not adjusted to pH 2, then the sample must be analyzed within seven days of sampling.
10 The pH adjustment is not required if acrolein will not be measured. Samples for acrolein receiving no pH adjustment must be analyzed within 3 days of sampling.
11 When the extractable analytes of concern fall within a single chemical category, the specified preservative and maximum holding times should be observed for optimum safeguard of sample integrity (i.e., use all necessary preservatives and hold for the shortest time listed). When the analytes of concern fall within two or more chemical categories, the sample may be preserved by cooling to ≤6 °C, reducing residual chlorine with 0.008% sodium thiosulfate, storing in the dark, and adjusting the pH to 6-9; samples preserved in this manner may be held for seven days before extraction and for forty days after extraction. Exceptions to this optional preservation and holding time procedure are noted in footnote 5 (regarding the requirement for thiosulfate reduction), and footnotes 12, 13 (regarding the analysis of benzidine).
12 If 1,2-diphenylhydrazine is likely to be present, adjust the pH of the sample to 4.0 ± 0.2 to prevent rearrangement to benzidine.
13 Extracts may be stored up to 30 days at <0 °C.
14 For the analysis of diphenylnitrosamine, add 0.008% Na2S2O3 and adjust pH to 7-10 with NaOH within 24 hours of sampling.
15 The pH adjustment may be performed upon receipt at the laboratory and may be omitted if the samples are extracted within 72 hours of collection. For the analysis of aldrin, add 0.008% Na2S2O3.
16 Place sufficient ice with the samples in the shipping container to ensure that ice is still present when the samples arrive at the laboratory. However, even if ice is present when the samples arrive, immediately measure the temperature of the samples and confirm that the preservation temperature maximum has not been exceeded. In the isolated cases where it can be documented that this holding temperature cannot be met, the permittee can be given the option of on-site testing or can request a variance. The request for a variance should include supportive data which show that the toxicity of the effluent samples is not reduced because of the increased holding temperature. Aqueous samples must not be frozen. Hand-delivered samples used on the day of collection do not need to be cooled to 0 to 6 °C prior to test initiation.
17 Samples collected for the determination of trace level mercury (<100 ng/L) using EPA Method 1631 must be collected in tightly-capped fluoropolymer or glass bottles and preserved with BrCl or HCl solution within 48 hours of sample collection. The time to preservation may be extended to 28 days if a sample is oxidized in the sample bottle. A sample collected for dissolved trace level mercury should be filtered in the laboratory within 24 hours of the time of collection. However, if circumstances preclude overnight shipment, the sample should be filtered in a designated clean area in the field in accordance with procedures given in Method 1669. If sample integrity will not be maintained by shipment to and filtration in the laboratory, the sample must be filtered in a designated clean area in the field within the time period necessary to maintain sample integrity. A sample that has been collected for determination of total or dissolved trace level mercury must be analyzed within 90 days of sample collection.
18 Aqueous samples must be preserved at ≤6 °C, and should not be frozen unless data demonstrating that sample freezing does not adversely impact sample integrity is maintained on file and accepted as valid by the regulatory authority. Also, for purposes of NPDES monitoring, the specification of “≤ °C” is used in place of the “4 °C” and “<4 °C” sample temperature requirements listed in some methods. It is not necessary to measure the sample temperature to three significant figures (1/100th of 1 degree); rather, three significant figures are specified so that rounding down to 6 °C may not be used to meet the ≤6 °C requirement. The preservation temperature does not apply to samples that are analyzed immediately (less than 15 minutes).
19 An aqueous sample may be collected and shipped without acid preservation. However, acid must be added at least 24 hours before analysis to dissolve any metals that adsorb to the container walls. If the sample must be analyzed within 24 hours of collection, add the acid immediately (see footnote 2). Soil and sediment samples do not need to be preserved with acid. The allowances in this footnote supersede the preservation and holding time requirements in the approved metals methods.
20 To achieve the 28-day holding time, use the ammonium sulfate buffer solution specified in EPA Method 218.6. The allowance in this footnote supersedes preservation and holding time requirements in the approved hexavalent chromium methods, unless this supersession would compromise the measurement, in which case requirements in the method must be followed.
21 Holding time is calculated from time of sample collection to elution for samples shipped to the laboratory in bulk and calculated from the time of sample filtration to elution for samples filtered in the field.
22 Sample analysis should begin as soon as possible after receipt; sample incubation must be started no later than 8 hours from time of collection.
23 For fecal coliform samples for sewage sludge (biosolids) only, the holding time is extended to 24 hours for the following sample types using either EPA Method 1680 (LTB-EC) or 1681 (A-1): Class A composted, Class B aerobically digested, and Class B anaerobically digested.
24 The immediate filtration requirement in orthophosphate measurement is to assess the dissolved or bio-available form of orthophosphorus (i.e., that which passes through a 0.45-micron filter), hence the requirement to filter the sample immediately upon collection (i.e., within 15 minutes of collection).
Cite this article: FindLaw.com - Code of Federal Regulations Title 40. Protection of Environment § 40.136.3, (b) to end Identification of test procedures - last updated January 02, 2025 | https://codes.findlaw.com/cfr/title-40-protection-of-environment/cfr-sect-40-136-3-nr2/
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